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Advancing Effortless Multiplex Western Blot Imaging

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Product Name: Advancing Effortless Multiplex Western Blot Imaging
Product Type: FluorChem M MultiFluor System
Exhibitors: ProteinSimple
Product Documentation: No Related Documents

Brief

Advancing Effortless Multiplex Western Blot Imaging FluorChem M sets a new standard for quantitative multiplex Western blot and gel imaging.


Advancing Effortless Multiplex Western Blot Imaging  A detailed description of
Advancing Effortless Multiplex Western Blot Imaging FluorChem M sets a new standard for quantitative multiplex Western blot and gel imaging. Its unique, compact design incorporates state-of-the-art CCD optical technology with an intuitive touchscreen interface for effortless detection of multicolor fluorescent, chemiluminescent, UV-excited and colorimetric gels and blots. Combining three key elements — FluorChem M takes the complication out of imaging from start to finish. No darkroom, no training, no computer required.


Clearly Quantitative
Multiplex Fluorescent Detection
FluorChem M provides a complete, quantitative solution for Western blot detection and analysis. Direct multicolor fluorescent detection is a stable, non-enzymatic approach that offers greater linearity and dynamic range — resulting in superior band clarity and proportional quantitative accuracy.
 
Serial dilutions of Transferrin from 1.0 μg to 0.5 pg were detected by Western blot using MultiFluor Green secondary antibody. The quantitative linear range was above 3 orders of magnitude with a 0.5 pg limit of detection.

Serial dilutions of Transferrin were detected by Western blot using MultiFluor Green secondary antibody. The dynamic range of detection was approximately 5 logs.

Identify Co-Migrating Proteins
Proteins, such as native and phosphorylated isoforms, that 
overlap due to similar migrating patterns can be probed on
the same blot. Image data from each fluorescent channel can 
be overlaid or viewed independently to readily detect subtle 
mobility shifts and resolve overlapping bands.
 

HeLa lysates probed with anti-ERK1/2 and anti-pERK1/2 primary antibodies followed by MultiFluor Green and MultiFluor Red secondary antibodies.

Detect Multiple Proteins Simultaneously
Save time and precious samples by detecting multiple proteins on the same blot. The need to strip and reprobe is eliminated, resulting in higher data integrity and shorter assay times. Band intensity values can be normalized to another
protein or loading control to correct for inaccuracies.

HeLa lysates at 4 μg, 1 μg and 0.25 μg probed with anti-Hsp-70, anti-β-Actin and anti-ERK1/2 primary antibodies followed by MultiFluor Green, MultiFluor Blue, and MultiFluor Red secondary antibodies.

Decidedly Uncomplicated
DIGITAL DARKROOM Software
Are complicated imagers keeping you in the darkroom?Advancing Effortless Multiplex Western Blot Imaging  FluorChem M’s integrated computer and intuitive touchscreen interface make imaging easy.
Step 1: Choose Your Application Select from a library of optimized protocols.


Step 2: Capture Your Image One-touch acquisition includes instant post-capture image optimization.


Step 3: Review Your Results Images are automatically saved and can be immediately printed or copied.

Multi-Image Overlay
Advancing Effortless Multiplex Western Blot Imaging  FluorChem M’s image overlay capabilities let you easily review and compare data.
• Display multichannel fluorescent images in multicolor overlay, single color or grayscale.
• Overlay molecular weight markers on Western blots for simultaneous viewing.
• Monitor chemiluminescent exposures taken at multiple time points.

Breaking Film Boundaries
Chemiluminescent Imaging
Escape the darkroom and experience how effortless imaging can be on FluorChem M. With faster imaging speeds and a linear range that surpasses film, crucial data are not lost due to under or overexposure. Load less protein, use less antibody, and never miss a faint band again.

Automated Digital Darkroom
Take the guesswork out of imaging. FluorChem M’s acquisition process automatically determines the optimal exposure and accurately captures the blot’s entire signal range. Comparative viewing of virtual exposures eliminates the hassle and expense of taking multiple film exposures.

Remote Monitoring and Image Access
Monitor acquisition progress and access images on FluorChem M from any networked computer or Wi-Fi enabled device. Our unique browser-based remote interface simplifies image retrieval and is ideal for multi-lab environments.




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